Identification and Polymorphism Analysis of EST-SSR Molecular Markers in Solanumm elongena L.
ZENG Meijuan1,2, LIU Jianting1,2, ZHUO Lingling1,2, CHEN Mindong1,2, YE Xinru1,2, WANG Bin1,2, ZHU Haisheng1,2*, WEN Qingfang1,2*
In order to speed up the molecular genetic research and molecular marker-assisted breeding of Solanumm elongena L., transcriptome sequencing was carried out. The software MISA (MicroSAtellite) was used to search for SSR loci in Solanumm elongena L. transcriptome. A total of 5 562 SSR loci were detected, distributed in 3 438 Unigene, with a frequency of 13.23% and an average distribution distance of 9.73 Kb. The major repeat motifs were trinucleotide and dinucleotide, which accounted for 63.75% (3 546) and 22.83% (1 270), respectively. Primers were designed by Primer 3.0, then 100 pairs of primers with more than 20 bp SSR sequences were randomly selected for synthesis, 92 pairs of primers were amplified effectively, accounting for 92% of 100 pairs of SSR primers. Thirty pairs of primers were randomly selected from effective amplification primers to amplify 29 Solanumm elongena L. germplasms for the polymorphism analysis. 30 pairs of primers were polymorphic. Through UPGMA mapping, 29 different Solanumm elongena L. samples were divided into two categories. The EST-SSR markers based on Solanumm elongena L. transcriptome sequencing have high availability and can provide more abundant marker sources for Solanumm elongena L. germplasm identification, genetic relationship analysis and genetic map construction.
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