Effect of HCMV Infection on Energy Metabolism of Monocytes
HUANG Kaizhao1,2,3, FANG Yangyang1,2,3, XU Xi1,2,3, GUO Binhan1,2,3, ZHENG Xiaoqun1,2,3*
The paper aims to investigate the effect of HCMV (human cytomegalovirus) infection on energy metabolism of human monocytic leukemia cell line THP-1. Flow cytometry and hippocampal bioenergy analyzer were used to detect the cell survival rate, OCR (oxygen consumption rate), and ECAR (extracellular acidification rate) after HCMV infection with THP-1. Mitochondrial kinetics-related proteins were identified by Western blot, and the expression levels of cell metabolism-related genes and proteins were detected by RNA-seq and mass spectrometry. The results showed that after HCMV infection for 24 h, 48 h and 72 h, the level of oxidative phosphorylation of mitochondria increased and the level of glycolysis decreased (P<0.05). The expression of mitochondrial fusion proteins MFN1, OPA1, and OMA1 in the infection group was considerably higher than that in the control group (P<0.05). The expression of oxidative phosphorylation-related genes such as ATP8A1, ATP6AP1L, and ATP6V1C2 was significantly up-regulated (P<0.001), while the expression of glycolysis-related genes such as LDHA, ENO3 and ENO1 was significantly down-regulated (P<0.001). Mass spectrometry analysis showed that the expression of metabolism-related proteins such as ATP6V1A, ATP5O, and PGP in the infected group was significantly up-regulated (P<0.01). Our study demonstrate that HCMV infection may promote oxidative phosphorylation and inhibit glycolysis by inducing mitochondrial fusion and regulating the expression of genes and proteins related to energy metabolism in THP-1 cells.
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