Interleukin-6 Regulates Proliferation of Human Umbilical Vascular Smooth Muscle cells via STAT3/Oct-1/ATM Pathway
LONG Caifeng, HAN Haoru, DENG Weiyan, SUN Jianle, ZHANG Ping*
This study aimed to explore the effect of IL-6 on the proliferation of HUVSMCs (human umbilical vascular smooth muscle cells) and classify one of its molecular pathway. Cell proliferation was examined with CCK-8 (cell count kit-8) and EdU (5-ethynyl-2-deoxyuracil riboside) imaging assay. The protein expression level was tested with Western blot. Oct-1 (octamer transcription factor-1) expression was inhibited using small interfering RNA, and the position of Oct-1 in cells was detected with immunofluorescence microscopy. It was found that, firstly, IL-6 promoted HUVSMCs proliferation and viability apparently. Secondly, IL-6 activated phosphorylation of STAT3 (signal transducer and activator of transcription 3) at Ser727, and enhanced the expression of Oct-1 and ATM (ataxia telangiectasia mutated). Thirdly, Oct-1 was mainly concentrated in the nucleus when IL-6 stimulated cells at 0 h and 6 h, and part of Oct-1 transferred to the cytoplasm at 12 h and 24 h. Finally, inhibition of Oct-1 reduced cell proliferation and decreased the expression of IL-6 and ATM. Adding IL-6 reversed the proliferation of HUVSMCs inhibited by Oct-1. In conclusion, the results classify that IL-6 induces the proliferation of HUVSMCs through the STAT3/Oct-1/ATM pathway. This study provides a new clue for the mechanical treatment of cardiovascular disease.
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