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The Inhibitory Effects of Anti-P-selectin Lectin-EGF Monoclonal Antibody on Maturation and Function of Human Dendritic Cells
Tong Zhou*, Gui-Zhi Sun, Yu-Mei Zhang, Ya-Peng Zhao, Wu-Kai Yin,Yan-Yun Zhang1, Dong-Qing Zhang1, Nan Chen
Department of Nephrology, Ruijin Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 200025, China;1Shanghai Institute of Immunology, Medical School of Shanghai Jiao Tong University, Shanghai 200025, China
Abstract: To observe the effects of anti-P-selectin lectin-EGF monoclonal antibody (PsL-EGFmAb) on maturation and function of human dendritic cells (DCs) and discuss its mechanism. CD34+ stem cells were isolated from cord blood and multiplied by SCF， GM-CSF， TGF-β1， Flt-3L， TNF-α culture in vitro， PsL-EGFmAb was added during DCs maturation. FACS was performed to detect the expression of CD1a， CD11c， CD83， CD80， CD86 and DC-SIGN (CD209); NF-κBp50， NF-κBp65 mRNA level was detect by RT-PCR; MTT was performed to measure T cell proliferation and ELISA was performed to determine IL-12p70 amount. PsL-EGFmAb had an inhibitory effect on C-type lectin DC-SIGN of DCs. It also inhibited NF-κBp50， NF-κBp65 mRNA， CD11c， CD83， CD80 and CD86 expression， reduced T cell proliferation stimulated by DCs in vitro and down-regulated IL-12p70 secretion. Totally， PsL-EGFmAb had an inhibitory effect on DCs maturation through NF-kB signaling pathway and such inhibition might relate to DC-SIGN， the pattern receptor and functional molecule of DCs.
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