Inhibition of Cyclin E Expression and Cell Proliferation by Small Interfering RNA in Breast Cancer MCF-7 Cell Li

Abstract: To study the inhibition of cyclin E expression by small interfering RNA (siRNA)， and alters cell proliferation in breast cancer MCF-7 cell line. The siRNA targeting cyclin E was chemically synthesized and transfected into MCF-7 cells by Oligofectamine， the expression of cyclin E was analyzed by quantitive PCR and Western blot， cell growth inhibition was measure with CCK-8 assay， cell cycle of the transfected cells was examined by flow cytometry， and cell colony forming ability was measured by soft-agar colony formation assay. After MCF-7 cells were transfected with 10， 50， 100 nmol/L siRNA， the expression of cyclin E mRNA was respectively suppressed with inhibition rate of 24.7%， 62.5% and 71.0%， the corresponding decrease of cyclin E protein expression was confirmed; cells in G1 phase increased after transfecting with siRNA-cyclin E， but cells in S phase decreased; lacking cyclin E expression exhibited significantly inhibited cell proliferation and showed less colony forming ability in the breast cancer cell line MCF-7 Cells. These results indicate that siRNA-cyclin E was a useful tool for silencing cyclin E and inhibiting cell proliferation in breast cancer cell line.
    
    

CSTR: 32200.14.cjcb.2006.02.0015