Exploration of the Designed Experiment of Cell Biology— Drug Resistance Analysis of Tumor Cells

Multi-drug resistance is the main reason for the failure of tumor chemotherapy in clinic, and its mechanism has been studied for many years. Drug resistance analysis of tumor cells is the basic work to study the mechanism of drug resistance. Its experimental ideas and methods are diverse and mature. During the open experimental teaching mode, students are encouraged to analyze the drug resistance of wild-type human breast cancer MCF-7/WT cells and adriamycin-resistant human breast cancer MCF-7/ADM cells from the aspects of cell morphology, drug sensitivity and expression of drug-resistant related proteins. First, students conduct independent research and design experimental programs in groups, then practice in the open undergraduate experimental teaching platform, finally summarize and evaluate experiments through diversified assessment modes. This paper presents the results through a specific embodiment, and makes a practical summary and reflection from the three aspects of overall scheme design, result statistics and after-class satisfaction survey. The whole experimental system is mature, safe and stable, with certain innovation, practicability and operability, which plays an important role in cultivating students’ initiative and creativity, improving their comprehensive experimental ability.

CSTR: 32200.14.cjcb.2020.04.0015