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Effects of Lipoxin A4 on Lipopolysaccharide-induce Oxidative Damage in Macrophage
Xiao-Yan Zhou1,2, Fang-Yun Xu1, Zhen-Yu Cai1, Hong-Mei Wang1, Zi-Rui Zhu1, Ping Wu2, Du-Yun Ye2*
1Department of Pathophysiology, Medical College of Nanchang University, Nanchang 330006, China; 2Department of Pathophysiology, Tongji Medical College, Huazhong University of Science &Technology, Wuhan 430030, China
Abstract: To study the effects of lipoxin A4 (LXA4) on oxidative damage of macrophages exposed to lipopolysaccharide (LPS)， the cells were exposure to LPS and different concentrations of LXA4. Then， the cell activity was analyzed by MTT assays; reactive oxygen species (ROS) were quantified through flow cytometry (FCM); the levels of nitric oxide (NO) and malondialdehyde (MDA)， besides the activities of SOD， GSH-Px， CAT， and iNOS were all detected through assay kits. In this study， the data indicated LXA4 was able to increase the survival activity of LPS-treated macrophages; decrease the levels of ROS， NO and MDA; inhibit the activity of iNOS; promote the activity of SOD， GSH-Px， and CAT. It seemed that LXA4 could antagonize LPS-induced oxidative damage through decrease the production of ROS and NO but also increase the activity of antioxidative enzymes.
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