Application of Mass Cytometry in Single-Cell Detection
YANG Yu, SUN Chuanqiang, GONG Zishan, YANG Ru, WANG Yan*
Mass cytometry is a cytometric technique in which multiple biomarkers of a single cell are detected through mass spectrometry. It can simultaneously measure more than 50 biomarkers at single-cell resolution, effectively augmenting the ability to evaluate cell growth processes and complex cellular systems. This work will briefly introduce the basic principle of mass cytometry and discuss metal labeling, mass analyzer and high-dimensional single-cell data processing. The needs to design new metal tags and select time-of-flight mass spectrometry are clarified, the algorithms which can be used for high-dimensional single-cell data analysis are described. At the same time, the advantages and limitations of these algorithms are discussed.
CN
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