The Role of Single-Cell Sequencing in Stem Cell
LIU Fangyuan, SU Xiulan*
Cellular heterogeneity is a fundamental characteristic in biological tissues and easily influenced by external stimulus. Even homologous cells could vary and express different genes when growing environment changes. Stem cell is a class of special cells with unlimited potential of self-renew and differentiation, acting as an essential role in the development of embryonic tissues and the dynamic balance in biological tissues. Single-cell sequencing technology provides a powerful tool for analyzing cellular heterogeneity in a more accurate way. In this review, we will describe the development of single-cell sequencing technology in recent years, including singlecell separation, genome amplification and sequencing analysis, and discuss the applications in multi-potential stem cells, tumor stem cells and tissue-specific stem cells.
CN
EN