Effects of Combination of Ghrelin and Arsenic Trioxide on the Proliferation and Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells
ZHOU Liutao, ZHU Xuemin, DENG Wen*
This work was to investigate the effects of Ghrelin on proliferation and osteogenic differentiation of BMSCs (bone marrow mesenchymal stem cells) induced by As2O3 (arsenic trioxide). BMSCs were divided into four groups: control group, As2O3 group, Ghrelin group and As2O3+Ghrelin group. MTT assay was used to detect cell proliferation. Real-time PCR and Western blot were used to detect the levels of OPN, ALP and RUNX2 mRNA and protein in BMSCs of different induction groups at day 7, 14 of osteogenesis induction. Alizarin red staining was used to analyze the calcium salt deposition of cells in different induction groups at day 21 of osteogenesis induction. The results showed that the cell proliferation ability of Ghrelin group>control group>combined group>As2O3 group. Compared with the control group, As2O3 group significantly decreased the mRNA and protein expression of osteogenesis-related factors OPN, ALP and RUNX2 (P<0.05). There was no significant change in OPN protein expression on the 14th day in Ghrelin group, and the other factors were up-regulated (P<0.05). Compared with As2O3 group, the expression of 14th day of OPN and 7th day of ALP in the combined group had no significant difference, and the other factors were up-regulated (P<0.05). Calcium deposition: Ghrelin group>control group>combined group>As2O3 group. The results showed that 0.5 μmol/L As2O3 could inhibit the proliferation and osteogenic differentiation of BMSCs, while 600 ng/mL Ghrelin could promote the proliferation and osteogenic differentiation of BMSCs. And Ghrelin could weaken the inhibition of proliferation and osteogenic differentiation of BMSCs induced by As2O3 to some extent.
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