UHRF1 Promotes Proliferation, Invasion and Migration of Papillary Thyroid Carcinoma Cells by miR-206 Regulated ERα
XU Linwan, LIU Geli, GOU Xi, QIU Yibo, LIU Zhimin*
The aim of this study was to investigate the effects of UHRF1 (ubiquitin-like with PDH and ring finger domains 1) on proliferation, invasion and migration of PTC (papillary thyroid carcinoma) cells. The expression of UHRF1 mRNA, miR-206 and ERα mRNA in normal thyroid cells Nthy-ori3-1, thyroid papillary carcinoma cells BCPAP and K1 were detected by Real-time PCR. Real-time PCR was adopted to detect the expression of miR-206 and ERα mRNA after UHRF1 overexpression or interference treatment. After treatment of cells with overexpressed or interfered UHRF1, proliferation was examined by MTT while invasion and migration were determined by Transwell. Western blot and dual-luciferase reporter assay were used to verify the relationship between miR-206 and ERα. The results showed that the expression of UHRF1 mRNA and ERα mRNA in BCPAP and K1 cells was significantly higher than that in Nthy-ori3-1 cells (P<0.05), while the expression of miR-206 was significantly lower than that in Nthy-ori3-1 cells (P<0.05). After the cells were overexpressed or interfered by UHRF1, the expression level of miR-206 showed the opposite trend, while the expression level of ERα showed the same trend. UHRF1 promoted the proliferation, invasion and migration of Nthy-ori3-1, BCPAP and K1 cells. Western blot and dual-luciferase reporter assay confirmed that miR-206 targets ERα gene and inhibits its expression. Taken together, UHRF1 may promote the proliferation, invasion and migration of thyroid papillary carcinoma cells
through the regulation of ERα expression by miR-206.
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