Betulin on Anti-Hepatocellular Carcinoma through SREBP1 Inhibits the Target Gene PIK3R3

LUO Ji^1#, MIAO Duling^2#, CHENG Zhujun², WEN Yusong², WEN Heyi², WANG Zang², FU Fen¹, TANG Xiaoli³, HE Xiaoju^1*

(¹The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China; ²Institute of Translational Medicine of Nanchang

Abstract:

This study focused on the molecular mechanism of betulin on Anti-hepatocellular carcinoma through SREBP1 inhibition of target gene PIK3R3. Cell proliferation was detected by MTT assay. Cell cycle and apoptosis were detected by flow cytometry. mRNA expression levels of cyclins, cyclin dependent kinase and apoptosis-related genes were detected by qRT-PCR. SREBP1-regulated apoptosis-related genes were screened by integration of RNA-seq and ChIP-Seq data. The regulation of SREBP1 on the apoptotic gene was detected by Dual-Luciferase reporter system. The results showed that betulin can significantly inhibit the proliferation of hepatocellular carcinoma cells, induce apoptosis, and block hepatocellular carcinoma cells in G2/M phase. Betulin can directly down-regulate the expression of apoptosis gene PIK3R3 to promote apoptosis and inhibit the proliferation of Hepatocellular Carcinoma cells.

CSTR: 32200.14.cjcb.2019.12.0006