Study on the Role of Caveolin-1 in EMCV Infected Host Cells

LIU Yang^1,2, ZHAO Kexue^1,2, XU Shujuan^1,2, LIU Yizhong^2*， LI Qiongyi^1,2*

(¹Key Bio-engineering and Technology Laboratory,Biomedical Research Center of the Northwest Minzu University, Lanzhou 730030, China; ²Life Science and Engineering College of Northwest Minzu University, Lanzhou 730030, China)

Abstract:

EMCV (encephalomyocarditis virus) is an important zoonotic pathogen, but its pathogenesis is not clear yet. Caveolin-1 mediate various viruses to infect host cells. In order to explore the effect of Caveolin-1 in EMCV-infection in vitro, this study detected the expression of Caveolin-1 at different times of EMCV-infection. And conducted the test of transient overexpression, transient silencing and inhibiting Caveolin-1 endocytic pathway on HeLa cells and HEK-293 cells, then observed the effects of viral infection. The results showed that the expression of Caveolin-1 in HeLa cells increased during virus infection. In the results of Caveolin-1 transient overexpression test, virus titer, virus copies and virus protein detection results showed that EMCV infection was promoted, the results of Caveolin-1 transient silencing test and inhibition test showed that EMCV infection was inhibited. These findings suggest that the Caveolin-1 dependent endocytosis pathway promotes EMCV infected host cells.

CSTR: 32200.14.cjcb.2019.12.0001