Effect of Thiomyristoyl on Several MLL-r Leukemia Cells
WU Yuanyuan, LI Jing*
TM (thiomyristoyl) is a highly specific Sirt2 inhibitor that is currently known to selectively inhibit the activity of certain cancer cells and exhibit significant anticancer effects. In this study, we used TM to treat several human MLL-r leukemia cells, and the acetylation level of H4K16 in cells was detected by Western blot, and compared the treated cells with cell functional experiments. Including the detection of clone formation ability, the detection of cell viability by MTT method, the detection of cell cycle and apoptosis by flow cytometry. At the same time, the effects of the decrease of Sirt2 expression on TM treatment was detected by constructing Sirt2 knockdown cell line and MTT assay. The results showed that TM could inhibit the growth of leukemia cells and reduce the acetylation level of H4K16 protein which is the acetylation substrate of Sirt2. At the same time, TM could inhibit cell cycle and promote cell apoptosis. The effect of the decrease in Sirt2 expression on TM treatment by MTT assay confirmed that the inhibition of TM on cells was related to the expression of Sirt2 in cells. This study demonstrates that TM can inhibit the proliferation of MLL-r leukemia cells and explore its inhibitory mechanism, which has important research significance for the treatment of MLL-r leukemia with TM.
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