The Inhibition and Mechanism of microRNA-31-3p in the Proliferation And Migration of Rhabdomyosarcoma Cells
CHEN Xiaoyan*, WANG Jiao, ZHAO Yunping, ZHONG Jiayun
This paper investigated the level of microRNA-31-3p (miR-31-3p) and the influence of proliferation and migration caused by miR-31-3p in rhabdomyosarcoma cells. Quantitative RT-PCR was performed to determine the level of miR-31-3p in rhabdomyosarcoma cells and straited muscle specimens. miR-31-3p was transfected into rhabdomyosarcoma cells with lipofectamine. The transfected cells were detected the proliferation, growth, migration and cell cycle by MTS assay, clone formation experiments, xCELLigence analysis and flow cytometry, respectively. The target was confirmed by luciferase activity assay and Western blot. Down regulation of STAT3 with siRNA is used to investigate the proliferation and migration influence of STAT3 in rhabdomyosarcoma cells. The results showed that the level of miR-31-3p in rhabdomyosarcoma cells was significantly down regulated compared with striated muscle specimens. Up regulated the level of miR-31-3p inhibited cell proliferation, clone formation and migration, and induced G1 arrest in rhabdomyosarcoma cells. The luciferase activity assay indicated that STAT3 was a target of miR-31-3p, and the Western blot showed that miR-31-3p could inhibit the level of STAT3 protein. Down regulated STAT3 inhibited the proliferation and migration of rhabdomyosarcoma cells RD. In conclusion, miR-31-3p inhibited the proliferation and migration in rhabdomyosarcoma cells probably through target STAT3.
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