High-Throughput Screening and Measurement Techniques in Synthetic Biology
CUI Jinming, LIU Chenli*
High-throughput screening and measurement techniques are receiving increasing attention in the “design-build-test-learn” closed loop of synthetic biology. In this paper, using the new activity detection of enzyme elements, the activity measurement of genetic circuits, the activity screening of natural products, and the study of the relationship between E. coli genotype and nematode host lifespan phenotype as examples, we introduced the common instruments and steps of high-throughput screening, as well as experimental design and analysis methods, to systematically sort out the relevant technology, and to provide reference and theoretical basis for the future progress of high-throughput screening technology.
CN
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