Progress in Gene Synthesis and Genome Editing

LIU Xiao¹, WANG Huiyuan¹, XIONG Yan¹, ZHAO Guoping², WANG Jin³^*

(¹Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; ²CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; ³College of Life Sciences, Shanghai Normal University, Shanghai 200234, China)

Abstract:

Synthetic biology is considered to be the next biotechnological revolution, following the discovery of the Double Helix and the Human Genome Sequencing Project, and is expected to bring about changes in many fields, such as industrial manufacturing, medicine, agriculture, environment and bioenergy. As the cornerstones of synthetic biology, DNA synthesis and gene editing technologies are the main driving force for the rapid development of synthetic biology. In this review, we focus on the main technologies and research progress in DNA synthesis and gene editing, including high-throughput gene synthesis using oligo pools and the CRISPR-mediated third-generation genome editing system.

CSTR: 32200.14.cjcb.2019.11.0002