Effects of 3D ECM Hydrogel on Behaviors of Cardiac Cells Derived from Stem Cells or Progenitor Cells
WANG Mingyu, LING Wenhui, XIONG Chunxia, XIE Dengfeng, CHEN Qiyu, CHU Xinyue, LI Yunxin, QIU Xiaoyan, LI Yuemin, XIAO Xiong*
Extracellular matrix (ECM) is a highly dynamic structure, which regulates the cellular proliferation, migration and differentiation etc. 3D hydrogel culture system created with cardiac ECM simulates microenvironment of heart in vivo, which promotes the maturation and functionalization of induced cardiomyocytes (iCMs) derived from stem cells or progenitor cells. ECM combines with 3D printed technology to promote the construction and applications of cardiac organoid. ECM patch also can be transplanted into damaged heart to improve its structure and functions. However, the efficiencies in repair of damaged heart with those strategies in vitro and in vivo are low, furthermore, the underlying mechanisms are poorly understood. Therefore, the composition, remodeling and functions of ECM, effects of histologic origins, biochemical compositions, physical properties and micro-vascular systems of 3D ECM hydrogels on behaviors of cardiac cells generated from stem cells or progenitor cells, applications of ECM on 3D printed technology and transplant in vivo for repairing the damaged heart were reviewed in this paper in order to lay the foundation for cell therapy, disease modeling, drug screening, exploration of development mechanism and so on.
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