Cloning and Expression Analysis of a Phytoenesynthase Gene in Hibiscus esculentus L.
LI Yongping1,2,3,4, CHEN Mindong1,2,3,4, ZHU Haisheng1,2,3,4*, WEN Qingfang1,2,3,4*, LIU Jianting1,2,3,4, LIN Siduan5
In this study, a PSY gene was cloned from Hibiscus esculentus L. by RT-PCR and RACE technique. The full-length cDNA sequence of PSY was 1 399 bp, which contained a 1 155 bp open reading frame (ORF) that encoded 384 amino acids with a predicted molecular weight of 43.45 kDa and a hypothetical isoelectric point (pI) of 8.729. The encoded protein had more than 90% homology with Gossypium arboretum, Gossypium hirsutum, Gossypium raimondii, Gossypium barbadense, and Theobroma cacao, which indicated that PSY gene of Okra was highly conserved. The gene was named HePSY and the GeneBank accession number was MG372369. Real-time PCR analysis revealed that HePSY could be expressed in different tissues of Hibiscus esculentus L., including roots, stems, leaves, flowers and fruits, and the level was the highest in mature leaves and 4 days after flowering during the process of leaf and fruit development, respectively. Carotenoid contents was explored. Hibiscus esculentus L. carotenoid was also the highest in mature leaves and 4 days after flowering during the process of leaf and fruit development respectively, which positive correlation with HePSY gene expression. These results revealed the expression of HePSY gene and the accumulation characteristics of carotenoid contents, and provided a base for the study of carotenoid molecular regulation mechanism in Hibiscus esculentus L.
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