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Cloning and Expression Analysis of LrANS from Lycium ruthenicum Murr

WANG Lianxing^1,2,3, MA Desen^1,2,3, SHI Guomin^2,3, GAO Sidan^1,2,3, GUO Jialei^1,2,3, ZHAO Weimin¹, LI Fengzhen^1,2, HE Tao^1,2,3*

(¹School of Ecol-Environmental Engineering, Xining 810016, China; ²Key Laboratory of Landscape Plants of Qinghai Province, Xining 810016, China; ³State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China)

Abstract:

Taking the Lycium ruthenicum Murr as materal, an anthocyanidin synthesis-related gene named LrANS (GenBank accession number MK713977) was cloned by homologous cloning and RACE methods. The sequence analysis showed that the length of LrANS gene was 1 527 bp, which contained a 1 290 bp ORF encoding 429 amino acids. Subcellular localization assays showed that the LrANS protein was localized in the cytoplasm and cytomembrane. Comparison of homology amino acid sequence indicated that LrANS had higher similarity with Nicotiana tabacum ANS (77.2%). The RT-PCR analysis showed that the LrANS gene was expressed in roots, stems, leaves, flowers, green fruits, purple fruits and black fruits, and the expression level in black fruits was significantly higher than other tissues. The LrANS gene expression was dramatically decreased under UV irradiation. It is speculated that the LrANS gene may play an important role in the anthocyanidin synthesis of L. ruthenicum.

CSTR: 32200.14.cjcb.2019.10.0010