Ankyrin Exposure Induced by Energy Exhaustion Triggers the Adhesion of Macrophages
WANG Yulin1,2, LONG Yaohang1, YE Yuannong2, ZHOU Houming2, CHEN Jin2, HUANG Jiangtao1, TANG Fuzhou2*
The aim of this paper is to study whether the energy exhaustion is associated with ankyrin exposure, and whether erythrocytes with ankyrin exposure adhere to macrophages. The energy exhaustion-induced protein kinace C (PKC) activation, ankyrin exposure, and the levels of intracellular calcium and ATP were separately tested. Results showed that the level of intracellular ATP decreased significantly after incubation for 2 h, and the energy exhaustion showed after incubation for 6 h. The treatment of erythrocytes with energy exhausting in a calcium-containing buffer led to ankyrin exposure and PKC activation. Moreover, ankyrin exposure induced erythrocyte adhesion to macrophages. Analyses of results suggested that the energy exhaustion induced calcium influx into the erythrocytes and PKC activation, ultimately leading to ankyrin exposure. This maybe provide a potential mechanism for studying the clearance of erythrocytes under the condition of energy depletion as well as insights into the molecular mechanisms of the removing of diseased erythrocytes induced by the energy exhaustion.
CN
EN