Preliminary Study on Conditional Reprogramming of Human Tumor Cells

The study used conditional reprogramming techniques to culture primary cells derived from human lung and breast cancer tissues. Cell count results showed that a large number of cells can be obtained in a short time in vitro using conditional reprogramming techniques and can be passaged in vitro for a longer period of time. Chromosomal specimens of the cultured primary cells were prepared and subjected to banding treatment, and the results showed that the chromosomes of the primary cells were polyploid. The STR analysis showed that the cultured cells substantially retained the molecular genetic properties of the tissue from which they were derived.HE staining showed significant polynuclear and distinct mitotic figures in the cultured primary cells. The results of immunohistochemistry showed that the primary cells cultured in vitro expressed CD44 and EpCAM, and did not express common surface markers such as ER and CK7. In vitro suspension culture, organ-like culture and flow cytometry results showed that the primary cells cultured by the conditional reprogramming technique had the characteristics of stem cells. In summary, the conditional reprogramming technique can obtain more primary tumor cells in vitro in a short time, and the tumor cells have the characteristics of partial stem cells.

CSTR: 32200.14.cjcb.2019.10.0004