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Study on Loading Platelets with Trehalose
Xin-Li Zhou*, Hong Zhu1, Shao-Zhi Zhang , Fa-Ming Zhu1, Guang-Ming Chen, Li-Xing Yan1
Cryobiology Laboratory, Institute of Refrigeration and Cryogenic Engineering, Zhejiang University, Hangzhou 310027, China; 1Blood Center of Zhejiang Province, Hangzhou 310006, China
Abstract: Introducing the impermeable lyoprotectant trehalose into the cytoplasm is the first step toward preserving platelets by freeze-drying. We investigated the loading of the human platelets with trehalose. Trehalose uptake was measured as a function of extracellular trehalose concentration， incubation time and incubation temperature. We found an effective uptake of the trehalose at 37 ℃ in a time span of 4 h in the presence of 50 mmol/L external trehalose. The internal trehalose concentration reached above 15 mmol/L. Morphological study， hematology analysis and annexin V binding assay showed that trehalose loaded platelets maintain the normal morphology and function of platelets. These data provide the foundation in developing a protocol for freeze-drying platelets.
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