Mineralizing Solution and Dexamethasone Enhance Receptor Activator for NF-kB Ligand Expression in Rat Bone Marrow Stromal Cells

Abstract: Receptor activator for NF-kB ligand (RANKL) was one of crucial factors regulating osteoclastic differentiation and activation， which express on bone marrow stromal cells (BMSCs). BMSCs cultivated with mineralizing solution (aMEM medium supplemented with 20%FBS， 10? mol/L dexamethasone， 10 mmol/L Na-b-glycerophosphate， 50 mg/ml L-ascorbic acid) can be induced to differentiation into osteoblasts. Dexamethasone was the major component of mineralizing solution. To investigate the effect of mineralizing solution and dexamethasone on RANKL expression， RANKL expressed on rat BMSCs cultivated with mineralizing solution for 48 h was detected by immunofluorescence staining and that expressed on the stromal MC3T3-G2/PA6 cell line cultivated with dexamethasone for 72 h was detected by Western blot. The results showed that mineralizing solution and dexamethasone enhanced RANKL expressed on murine BMSCs in short period. It was suggested that the mechanism of dexamethasone-reduced osteoclast formation may be indirectly related to RANKL change in BMSCs.

CSTR: 32200.14.cjcb.2006.01.0022