Metformin Inhibits Proliferation and Migration of H1299 Cells Through Interfering ERK Pathways

In this paper we investigated the effects of metformin on proliferation, migration and apoptosis of lung adenocarcinoma H1299 cells and its underlying mechanism. The cell morphology of H1299 cells treated with metformin was observed under microscope, and the effect of metformin on the migration of H1299 cells was observed by wound healing, the effect of metformin on the apoptosis of H1299 cells was detected by Annexin V/PI, and the effect of metformin on the proliferation of H1299 cells was detected by 5-acetylene-2’deoxyuracil (Edu) assay. The results show that metformin changed the morphology of H1299 cells and significantly inhibited the migration of H1299 cells while metformin could not induce the apoptosis of H1299 cells. Meanwhile, metformin inhibited the proliferation of H1299 cells. Further study shows that metformin could down-regulate the phosphorylation levels of ERK and MEK proteins. In addition, Metformin increased the expression of E-Cadherin and decreased the expression of FAK and vimentin. The results indicate that metformin inhibited the proliferation and migration of H1299 cells mainly by inhibiting ERK signaling pathway. Besides, inhibition of migration was caused by up-regulation of E-Cadherin and down-regulation of FAK and vimentin. The results provide reference for metformin in the prevention and treatment of lung adenocarcinoma.

CSTR: 32200.14.cjcb.2019.08.0015