Optimization and Application of Serum Exosome Extraction in Patients with Multiple Myeloma
Wei Xiaojing, Du Chenxing, Xu Jie, Yu Zhen, Liu Lanting, Qiu Lugui, Hao Mu*
Exosome extraction methods are diverse, and there is no unified standard at present. Each method has its own advantages and disadvantages. Therefore, this paper compared the two methods of exosome extraction in serum of multiple myeloma patients, and summarized a method suitable for the extraction of exosome in patients with small amount and precious specimens. The kit extraction method can obtain sufficient exosomes but introduce impurities. Due to the small amount of exosomes and excessive loss of exosomes, it is difficult to carry out subsequent experiments such as the extraction of RNA in exosomes, so it is difficult to obtain proteins or nucleic acids in exosomes. In this paper, the advantages of differential ultracentrifugation extraction method and kit extraction method were combined to summarize a more time-saving and convenient method with higher exosome purity.
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