Effects of Different Frequency Acoustic Wave Loading on Behavior and Expression of 5-Hydroxytryptamine, Dopamine and D2 Receptor in Hippocampus of SD Rats
Deng Zhidan, Ren Yang, Zhao Yajin, Chen Dong, Hou Man, Wang Xiang*
The aim of this study was to investigate the effects of different frequencies of acoustic wave loading on the behavior of depression, the secretion of 5-hydroxytryptamine (5-HT), dopamine (DA) and the expression of dopamine D2 receptor in the hippocampus of SD rats. This study focused on rhythm elements of music based on music analysis. We created various different rhythm (frequencies) sound waves with composition software Finale2011 for sound stimulation providing playable audio files. Taking the heart rate frequency of normal, anesthetized and stressed rats as standard, the audio files with a frequency of 350 beats/min, 300 beats/min, 400 beats/min were simulated and loaded into SD rats. The concentrations of 5-HT and DA in the left and right sides of the hippocampus were measured with a kit, and the expression of D2 receptor was analyzed by immunofluorescence intensity. The results showed that compared with the control group, the rats in the 300 beats/min group had more positive behavior changes, higher body weight, higher sugar-water preference rate, and higher concentrations of 5-HT and DA, and increased 0.098 8 ng/mL and 808.5 ng/mL respectively (P<0.01). The behavioral changes of 350 beats/min group and 400 beats/min group tended to be depressed, body weight and glucose-water preference rate decreased significantly. 5-HT and DA in 350 beats/min group decreased 0.005 3 ng/mL (P<0.01) and 308.5 ng/mL (P<0.01), respectively. In 400 beats/min group, 0.031 5 ng/mL and 1 067.5 ng/mL were decreased (P<0.01) and 1 067.5 ng/mL (P<0.01), respectively. In addition, the expression of D2 receptor was significantly enhanced by acoustic wave loading at the frequency of 300 beats/min, while the expression of D2 receptor was decreased in the other two groups. The results showed that different frequencies of acoustic loading have different effects on behavioral changes, body weight, glucose-water preference rate, 5-HT and DA secretion in SD rats, and the loading frequency is close to that of heart rate in anesthetized rats. It is also beneficial to the changes of hippocampus behavior and body weight, the increase of glucose and water preference, the secretion of 5-HT and DA, and the expression of D2 receptor.
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