Mechanism of miR-193a-5p on Migration and Invasion of Pancreatic Cancer Cells In Vitro
Li Manman, Yang Zhaocong, Lu Zhou, Pan Yi, Jin Liang*
The aim of this study was to investigate the effect and mechanisms of miR-193a-5p on migration and invasion of human breast cancer cells in vitro. Panc-1 cells were treated with miR-193a-5p mimic or miR-193a-5p inhibitor to up-regulate/down-regulate the expression level of miR-193a-5p. Wound-healing assay and Transwell chamber were employed to determine cell migration and invasion in vitro. The target gene of miR-193a-5p was predicted with the TargetScan 7.1 database and verified through luciferase reporter method. The effects of miR-193a-5p on the expression of the potential target were detected by Real-time PCR and Western blot. Results showed that in vitro migration and invasion ability of Panc-1 cells was increased significantly by Real-time PCR, which could target Prox1 in the 3′UTR region. The mRNA and protein expression of Prox1 was decreased by miR-193a-5p overexpression. The results validated that miR-193a-5p, highly expressed in pancreatic cancer, could down-regulate Prox1, which in turn promotes migration and invasion ability of breast cancer cells, thus promoting pancreatic cancer metastasis.
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