Preliminary Research on the Inhibitory Effect of Grape Seed Proanthocyanidins on Colon Cancer Cells

Objective grape seed proanthocyanidins (GSPs) are a kind of polyphenols commonly found in nature which are natural antioxidants. Recently, the relationship between antioxidants and cancer has attracted great attention. In this paper, colon cancer cell lines SW480 and SW620 were used as models to explore the effect of GSPs on the biological characteristics of colon cancer cells and the molecular mechanisms by which was investigated further. Methods MTT assay and flow cytometry were used to detect the effects of GSPs on cell proliferation, cell cycle and apoptosis. The genes related to GSPs regulation were screened by next-generation sequencing and the expression changes of target molecules were verified by real-time quantitative PCR and Western blotting. Results Both SW480 and SW620 cells were treated by GSPs, the biological characteristics were changed significantly, e.g.: cell morphological changed, the cell proliferation ability decreased, the cell cycle changed, and the apoptosis rate increased. The mechanism by which the Akt signaling pathway as well as antioxidant signaling pathway represented by FoxM1 plays an important role in this process. Conclusion GSPs can significantly affect the biological characteristics of colon cancer cells with which Akt signaling pathway and FoxM1 play important roles.

CSTR: 32200.14.cjcb.2019.08.0006