The Effects of Bone Marrow Derived Mesenchymal Stem Cells on the Expression of DNA Methyltransferase 1 in Keloid Fibroblasts
Xue Biyu, Xue Bin*
This article investigated the effects of bone marrow derived mesenchymal stem cells (BMSC) on the expression of DNA methyltransferase 1 (DNMT1) and related factor expression changes of TGF-β/Smad signal transduction pathways in keloid fibroblasts (KF). BMSC and fibroblasts were cultured, the indirect co-culture system using Transwell chamber with BMSC was established. The expression changes of DNMT1 were detected by immunofluorescence, Western blot and qPCR. Western blot assay was used to detect the protein expression of TGF-β1. qPCR was used to detect the expression of Smad7. Immunohistochemistry experiments showed that DNMT1 showed high expression in KF. Western blot and qPCR results showed that the expression of DNMT1 in co-cultured KF group was significantly reduced than that in the control group (P<0.01). The protein expression of TGF-β1 in co-cultured KF group was significantly reduced than that in the control group (P<0.01).Smad7 mRNA expression of co-culture KF group was significantly increased than the KF group (P<0.05). BMSC microenvironment could inhibit the expression of DNMT1 in co-cultured KF, thus inhibiting the KF by regulating TGF-β/Smad signal transduction pathway.
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