Promoted Role of Calcium Voltage-Gated Channel Auxiliary Subunit Beta 3 in the Osteogenic Differentiation of Mouse Adipose-Derived Stem Cells
Fu Xi, Wang Feng*
Mesenchymal stem cells(MSCs) play an important role in the treatment of bone-related diseases, but their mechanisms in osteogenic differentiation have not been fully elucidated. Here, Gene expression profiles related to vehicle-treated cells were assessed by microarray analysis. 22 genes were screened out based on their expression profiles, which were up-regulated in osteogenic differentiation, down-regulated in adipogenic differentiation and under tightly control of HDACi treatment. Interestingly, 6 genes (including Cacnb3, Lpcat2, Qpct, etc.) of these 22 genes are associated with calcium regulation, suggesting that calcium ions may play an important role in MSCs differentiation. In subsequent experiments, we observed that Cacnb3 and Lpcat2 were up-regulated during the osteogenic differentiation of several types of cells, including mouse adipose mesenchymal stem cells (ADSCs), human mesenchymal stem cells (hBMSCs) and MC3T3-E1 pre-osteoblasts. It is suggested that Cacnb3 and Lpcat2 might play an important role in the osteogenic differentiation of ADSCs and may promote osteogenic differentiation of ADSCs in vitro. In addition, we found that the intracellular calcium ion concentration was significantly lower than that of the control group after knocking down Cacnb3 with calcium ion fluorescence probe, and the expression of osteogenic related genes (ALP, Runx2) was significantly down-regulated, which indicated that Cacnb3 may affect the osteogenic differentiation of cells by the change of Ca2+ concentration. This study laid the foundation for further exploring the mechanism of osteogenic differentiation of mesenchymal stem cells.
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