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The Influence of Anti-inflammatory Agents upon the Phenotype and Proinflammatory Cytokine Secretion of Cultured Human Dendritic Cells
Tong Zhou*, Gui-Zhi Sun , Yu-Mei Zhang , Ya-Peng Zhao, Yan-Yun Zhang1, Dong-Qing Zhang1, Nan Chen
Department of Nephrology, Ruijin Hospital, Medical School of Shanghai Jiaotong University, Shanghai 200025, China;1Shanghai Institute of Immunology, Medical School of Shanghai Jiaotong University, Shanghai 200025, China
Abstract: To observe the influence of anti-P-selectin lectin-EGF domain monoclonal antibody (PsL-EGFmAb) and interleukin-10 (IL-10) upon the morphologic features of cultured human dendritic cells (DCs)， as well as IL-12 secretion. Cord blood CD34+ stem cells were isolated and cultured in 20% IMDM medium with SCF， GM-CSF， TGF-b1， Flt-3L， TNF-a. DCs were interfered by PsL-EGFmAb and IL-10. HLA-DR， CD1a， CD11c， CD83， CD80 and CD86 was assayed by flow cytometry. IL-12 p35， IL-12 p40 mRNA was detected by RT-PCR， IL-12 p70 secreted in culture medium was detected by ELISA. The expression of CD11c， CD83， CD80， CD86 was lower in PsL-EGFmAb and IL-10 groups than mature DCs group， and HLA-DR was lower in PsL-EGFmAb group compared with mature group. The mRNA level of IL-12 p35， IL-12 p40 and the secretion of IL-12 p70 was suppressed with PsL-EGFmAb or IL-10 treated， respectively. It showed PsL-EGFmAb and IL-10 might inhibit the expression of adhesion and co-stimulation molecules， and inhibit the secretion of proinflammatory cytokine. PsL-EGFmAb and IL-10 might adjust the antigen presenting function of DCs.
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