Research Progress on High-Throughput Automated SNP Detection Technology
Su Rui, Lin Jun, Chen Liqun*, Cai Weiwen*
As the third-generation molecular genetic marker, single nucleotide polymorphism (SNP) has received extensive attention due to its research importance in the fields of genetics and medicine. It is necessary to establish a high-throughput automated SNP detection technology. This paper briefly summarizes the principles, applications and characteristics of representative traditional SNP detection methods: single-strand conformation polymorphism and restriction fragment length polymorphism. A detailed overview of several representative highthroughput automated SNP detection methods: Sanger sequencing, pyrosequencing, MassARRAY (sequencing), gene chip, including their principles, applications and characteristics. Moreover, the way to the high-throughput automated genotyping in the future is briefly discussed.
CN
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