Vitamin D Inhibits The Proliferation of Gastric Cancer Cells via Down-Regulating the Expression of β-catenin Phosphorylation by VDR
Hu Qian1, Du Chao2, Dong Hui2, Zhang Bingqiang1*
To investigate the effect and molecular mechanism of vitamin D (VD) and vitamin D receptor(VDR) on the proliferation of gastric cancer cells, the first step is that we observed the expression of VDR in SGC-7901 and MKN-45 gastric cancer cells by immunofluorescence (IF) assay. Furthermore, VDR-shRNA stable cell lines of SGC-7901 and MKN-45 cells were selected by puromycin under using shRNA interfere with lentivirus and transfecting the gastric cancer cells. The effects of VD/VDR on the proliferation and cell cycle of two kinds of gastric cancer cells were detected by CCK-8 (Cell Counting Kit-8) assay, colony formation assay, flow cytometry and Western blot. Eventually, the effects of VD/VDR on the expression of β-catenin phosphorylation (p-β-catenin) of gastric cancer cells were tested by Western blot.The results showed that VDR was expressed in both MKN-45 and SGC-7901 cells.The proliferation and colony formation of gastric cancer cells were significantly inhibited, while the expression of p-β-catenin was inhibited in the presence of Osteotriol(1α,25(OH)2D3),but there were no significant effects on cell cycle distribution and the expression of cyclin D1. After down-regulation the expression of VDR, VD had no significant effect on the the expression of p-β-catenin. It confirmed that VDR can inhibit the proliferation of gastric cancer cells via inhibiting the expression of p-β-catenin.
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