Effects of Salvia Miltiorrhiza Injection on Proliferation and Differentiation of Neural Stem Cells Derived from Cerebral Cortex of Fetal Mice

Purpose of this study is to investigate the effects of Salvia miltiorrhiza injection on the proliferation and differentiation of neural stem cells (NSCs) derived from cerebral cortex of fetal mouse. The drug concentration was calculated from the concentration of crude drug of Salvia miltiorrhiza injection (1.5 mg/mL). The cortex neural stem cells were cultured in vitro and divided into control group and Salvia miltiorrhiza injection treatment group. The drug effects on the proliferation and differentiation of NSCs were detected by cell proliferation assay CCK-8, Brdu, confocal high-content analysis system and RT-PCR. Results of CCK-8 and BrdU detection showed that treating with 1.17 μg/mL of Salvia miltiorrhiza injection, the NSCs’ capacity of proliferation can be significantly improved than that of the control group (P<0.01); Confocal high-content analysis result indicated that the Salvia miltiorrhiza injection can apparently promote the differentiation of NSCs into neurons (P<0.01); RT-PCR results also demonstrated that the neuron-specific gene expression, such as NeuroD1 and Tuj1, increased significantly when NPC cells were pre-treated with Salvia miltiorrhiza injection. From the results, it can be concluded that Salvia miltiorrhiza injection can promote the proliferation of neural stem cells and also enhance their capacity of differentiation into neurons.

CSTR: 32200.14.cjcb.2019.07.0012