The Subcellular Localization of DJ-1 Protein in Dictyostelium discoideum
Chen Suwei*
The loss and mutations of DJ-1 were reported to cause the symptoms related to Parkinson’s disease (PD), but the role of DJ-1 in PD and its subcellular localization still remained controversial. Dictyostelium discoideum is one of the recognized models and can be used to study the neurodegenerative disease. DJ-1 protein in D. discoideum was labelled by green fluorescence protein (GFP) and its subcellular localization under basal and oxidative stressed conditions was observed using immunofluorescence and colocalization of DJ-1 and GFP in order to discover the possible links between DJ-1 subcellular localization and its mechanisms related to PD. The results showed that DJ-1 was predominantly cytosol under basal condition in D.discoideum, but translocated to the mitochondria with hydrogen peroxide. This translocation of DJ-1 could possibly be due to the oxidation of site C117, which provides a crucial clue in studying how DJ-1 protects the cells under oxidative stressed condition.
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