The Effect of Human β Defensin-2 on Proliferation, Migration, Invasion of Gastric Cancer SGC7901 Cells via TGF-β/Smad Signaling Pathway
Shi Yanmei1, Li Juan1, Liu Yan2, Chen Kang3, Zhou Lei1, Chen Xinnian1*
To study the effect of human β defensin-2 (hBD2) on the proliferation, migration, invasion of gastric cancer SGC7901 cells, eukaryotic expression vector pCMV-hBD2 are transfected onto human gastric cancer SGC7901 cells. The expression of hBD2, TGF-β1, p-Smad2/3, Smad2/3 and MMP9 were detected by Western blot. Transwell assay was evaluated migration and invasion of SGC7901 cells. EdU method and flow cytometry respectively were detected cell proliferation and cell cycle. The results showed that overexpression of hBD2, the expression levels of hBD2 in SGC7901 cells transfected eukaryotic expression vector pCMV-hBD2 were higher than that in SGC7901 and SGC7901-Blank cells transfected pCMV-Blank. The expression levels of TGF-β1, p-Smad2/3 and MMP9 in SGC7901-hBD2 cells all decreased, while the expression levels of Smad2/3 remained unchanged. At the same time, its migration, invasion and proliferation were inhibited and cell cycle G0/G1 phase was blocked. It is possibly that hBD2 regulates the migration, invasion and proliferation cycle of SGC7901 cells through downregulating the TGF-β/Smad signaling pathway.
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