Preparation of Nanoscale Bioactive Glass and the Effect on Proliferation of Fibroblast

Hu Qing¹, Li Yuli²*, Lin Cai³, Zhang Juanjuan³, Miao Guohou⁴

(¹School of Materials Science and Engineering, Jingdezhen Ceramic Institute, Jingdezhen 333403, China; ²Plastic Surgery Institute of Weifang Medical University, Weifang 261053, China; ³Burn-Wound Center, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; ⁴Guangzhou Institute of Oral Disease, Stomatology Hosptial of Guangzhou Medical University, Guangzhou 510140, China)

Abstract:

Nanoscale bioactive glass (NBG) was prepared using the sol-gel co-precipitation and freeze-drying methods in this study. Their morphology, dispersibility and average size were investigated by using SEM, TEM and laser particle analyzer, and the effects of their extract on the proliferation of human dermal fibroblasts (HDF) were studied by methyl thiazolyl tetrazolim (MTT) assay. The results showed that the NBG particle size was less than 50 nm, while the average particle size of NBG was 491.8 nm detected by Laser particle size analyzer which was less than that of sol-gel bioactive glass (SGBG), and the dispersibility of NBG was better than that of SGBG. Furthermore, MTT assay results showed that both SGBG and NBG extracts could stimulate the proliferation of HDF in appropriate concentration at 5, 10 and 15 times dilution. However, SGBG had better stimulating effect at the early culture time, while the NBG had more obvious effect at the late stage of the culture time. Therefore, NBG is more promising in wound repair.

CSTR: 32200.14.cjcb.2019.06.0010