Novel Nanomaterial CeO2NPs can Damage Ovarian Function by Inducing Cell Inflammation and Apoptosis
Yin Xin, Gao Rufei, He Junlin, Mu Xinyi, Chen Jun, Chen Xuemei, Liu Xueqing, Wang Yingxiong, Geng Yanqing*
CeO2NPs, a novel nanomaterial, has been wildly applicated in many fields, but its biosecurity is unknown. This study focused on the effect of CeO2NPs on the structure and function of ovary in rat. H&E staining and IHC showed that there were no obviously changes in ovary visceral index and ovarian structure between control group and CeO2NPs groups. According to the results of ELISA and Real-time PCR, the level of the key enzymes which regulate the synthesis of hormone were significantly increased in a dose dependent manner in response to CeO2NPs (P<0.05). Compared with the control group, the expression of FSHR and LHR were considerably decreased in the rat ovaries of CeO2NPs groups(P<0.05). Further study showed that the expression of inflammation related genes TNF-α, IL-6, IL-1β were remarkably increased (P<0.05) and anti-inflammation related protein IL-10 was significantly declined in rat ovaries of CeO2NPs groups (P<0.05). Furthermore, the expression of apoptosis related proteins caspase 3 and cleaved-caspase 3 in the rat ovaries were obviously increased (P<0.05), and the positive signal of TUNEL was stronger after CeO2NPs exposure. This study demonstrated that ovarian endocrine function might be damaged by CeO2NPs by inducing cell inflammation and apoptosis.
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