The Establishment and Development of Nuclear Transfer
Liao Zhaodi, Liu Zhen, Sun Qiang*
Nuclear transfer is a technique used to obtain clone embryos or individuals, in detail, people apply micromanipulation skills to transfer donor nuclei into enucleated oocytes, then the reconstructed embryos undergo either in vivo or in vitro culture. From 1950’s to the present, nuclear transfer technology has been widely and deeply developed, and play a significant role in the fields of life science. The establishment and development of nuclear transfer technology can be divided into two stages: initial stage started in the eggs of amphibians. In this period, nuclear transfer technique was firstly used to study the function of nucleus and its interaction with cytoplasm. This technique was further improved later in mammals. Compare with amphibians, the application of nuclear transfer technique in mammals is more intensive and extensive, and this mainly includes: clone of different mammalian species, clone with multiple types of cells, micromanipulation method and procedure improvement, reprogramming mechanism research and efficiency improvement of nuclear transfer, endangered species protection,personal stem cell therapy and gene-modified model generation. In this review we will discuss the development of nuclear transfer technology in the two stages and give a future prospect about the application of the technology in non-human primate model generation.
CN
EN