Isolation and Identification of Human Umbilical Cord Mesenchymal Stem Cells and Their Differentiation into Keratinocytes
Wei Ao, Zhang Dong, Liu Le, Liu Chunxia, Cao Junwei, Meng Fanhua, Zhang Yanru*
In this study, human umbilical cord mesenchymal stem cells (hUCMSCs) were divided from the umbilical cord matrix, purified by subculture, appraised by the expression of cell surface markers and differentiation abilities, and established hUCMSCs lines. The hUCMSCs expressed the transcription factor SOX2, mesenchymal stem cell surface marker genes CD29, CD90 and CD105, and did not express hematopoietic cell surface marker genes CD34, CD45 and human leukocyte antigen gene HLA-DR. They could be inducted to differentiate into neural cells, osteoblast and keratinocyte, demonstrating their multi-directional differentiation potential. After the keratinocyte induction, the differentiated cells expressed CK19 by immunostaining, and the expression of CK19 gene was gradually increased with time by PCR. In summary, this investigation established the hUCMSC cell line, which could induce differentiation system of keratinocytes, and provided basis for subsequent experiments.
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