Effects of Cinobufotalin on Cell Cycle of Human Gastric Cancer MKN-45 Cells

He Mengqi¹, Shao Shuli^1,2*, Feng Yuan¹, Jiao Kaihe¹, Zhang Weiwei^1,2, Zhang Zhenzhu^1,2, Cui Tingting¹, Li Tie¹

(¹College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihaer 161000, China; ²Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University, Qiqihaer 161000, China)

Abstract:

The study investigated the effects of cinobufotalin on the proliferation of human gastric cancer MKN-45 cell. The trypan blue exclusion was adopted to detect the effect of cinobufotalin on cell proliferation, the changes of cell morphology was observed under laser scanning confocal microscopy, the change of cell cycle were detected by FACS, and the expression of CCNB1, CCND1 gene mRNA and protein were tested by qRT-PCR and Western blot, respectively. The result showed that in a certain range, cinobufotalin inhibited the proliferation of human gastric cancer MKN-45 cell, which was dependent on time and dosage. After 48 h treatment with different concentrations of cinobufotalin on human gastric cancer MKN-45 cells, the cells showed obvious apoptosis characteristics, the decrease of G1 phase and the increase of S phase cells. Compared with the control group, the mRNA and protein expression of CCND1, CCNB1 significantly decreased (P<0.05). Together all, cinobufotalin can induce apoptosis, arrest cell cycle and inhibit cell proliferation of MKN-45 cells.

CSTR: 32200.14.cjcb.2019.04.0016