Effect of ABCA1 on Gene Expression Profiles of Trophoblast Cells

Du Haixia¹, Xie Chengmao¹, Zhang Jingjing², Lin li³*

(¹Department of Gynaecology, Beijing Obstetrics and Gynecology Hospital of Capital Medical University, Beijing 100026, China; ²Department of Delivery Room, Beijing Obstetrics and Gynecology Hospital of Capital Medical University, Beijing 100026, China; ³Department of Obstetrics and Gynecology, Perking University International Hospital, Beijing 102206, China)

Abstract:

This artical explored the effect of ABCA1 on human trophoblast function from the genetic perspective. The expression of ABCA1 in human trophoblast cells was regulated, and the changes of genes and related signaling pathways in trophoblast cells were detected by expression microarray, and the results were verified by Western blot and qRT-PCR. The results showed that when ABCA1 expression was up-regulated, 197 genes were up-regulated and 190 genes were down-regulated. When ABCA1 expression was down-regulated, 335 genes were elevated in expression and 459 genes were down-regulated. GO and KEGG analysis indicate that increased or decreased ABCA1 expression leads to changes in multiple signaling pathways in trophoblasts. Compared with the negative control group, the mRNA and protein levels of S1PR1 in the cells up-regulated by ABCA1 were significantly increased, while the mRNA and protein levels of CCL8, CXCL10 and CXCL11 were significantly decreased. The expression of ABCA1 was down-regulated after qRT-PCR and Western blot assay. The mRNA and protein levels of S1PR1 in the cells were significantly decreased, while the mRNA and protein levels of CCL8, CXCL10 and CXCL11 were significantly increased, which was consistent with the results of the chip. These results indicate that ABCA1 can affect trophoblast function by regulating multiple signaling pathways.

CSTR: 32200.14.cjcb.2019.04.0008