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The Expression of Nodal in Rat Ovarian Follicles at Different Developmental Stages
Li-Ping Yan, Chuang Li1, Bing-Ran Guo*
College of Life Science, Qufu Normal University, Qufu 273165, China; 1 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Abstract: To examine the expression and the role of Nodal in rat follicles at different stages of development. Pre-mature rats were injected with PMSG (10 IU/rat)， and ovaries were collected at 12， 24， 36 and 48 h after injection; Serial sections were prepared for each ovary; The structures of rat ovarian follicles were shown by HE staining after PMSG injection， and the localization of Nodal in rat ovaries was determined by immunohistochemistry. The results showed that Nodal was mainly distributed in granulosa cells close to the follicle cavity. It is suggested that Nodal may play a vital role in the formation of follicle cavity.
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