Knockdown of HMGB1 Influence the Migration and Invasion Abilities of Colorectal Cancer Cells
Long Luye, Wang Sixuan, Xu Jianxi, Fang Xuejiao, Qian Shihan, Lü Jianxin*
In this study, we investigated the effect of HMGB1 on migration and invasion in colorectal cancer cells. We constructed the colorectal cancer cell lines SW480 and HCT-15 with stable suppression of HMGB1. Transwell assay was used to detect cell migration and invasion. Western blot was used to detect protein expression. Transwell experiments showed that the migratory and invasive ability of shHMGB1 group was significantly greater than that of shNC group (negative control group) (**P<0.01, ***P<0.001). Western blot results showed that the epithelial marker ECadherin protein in the shHMGB group (knockdown of HMGB1group) was decreased compared with the shNC group (*P<0.05, **P<0.01). However, the mesenchymal marker, Snail protein expression level was increased (*P<0.05). In addition, the expression levels of c-Myc and GSK3B proteins in shHMGB1 group were significantly higher than those in shNC group (*P<0.05, **P<0.01, ***P<0.001). The results indicated that suppression of HMGB1 expression significantly promoted the migration and invasion of colorectal cancer cells as well as epithelial mesenchymal transition, providing new potential targets and new perspectives for the treatment of colorectal cancer.
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