Home > Browse Issues > Vol.41 No.3
In Vitro 3D Culture of Esophageal Squamous Cell Carcinoma
Liang Yabing1, Zhang Man2, Du Hua3, Yang Ling1*
1Clinical Medical Research Center, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China; 2Department of Thoracic Surgery, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China; 3School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010050, China
Abstract: The fresh esophageal squamous cell carcinoma tissue was minced into tiny pieces and mixed with matrigel， the mixture was cultured in the 96-well plate. The cultured mixture was taken out at the 2nd， 4th， 6th， 8th， 10th， 12th， 14th， 16th， 18th day， and fixed， embedded in paraffin. The sections were stained with hematoxylin and eosin， and performed immunohistochemistry with Ki-67， P63， P40 and cytoheratin5/6 antibody. HE staining showed that the cultured tumor tissue was basically the same as the original tissue. However， with the extension of culture， tissue blocks became small and tumor tissue showed atrophy and degeneration. The expression of P40 and P63 protein in tumor tissues decreased with the increase of culture time in vitro. The intensity and ratio of Ki- 67 protein in tumor tissues decreased with the increase of culture time. In conclusion， part of the characteristics of esophageal squamous cell carcinoma changes with the extension of the culture time in vitro， therefore， the shorter the time tissue cultured in vitro.
CN
EN