The Application of Single Cell Technologies in Stem Cell Tissue Repair and Drug Development

Abstract: The rapid advancement of single cell technologies in recent years break the limit of traditional biomedical research from studying mixed cell population to a single-cell level， thus solving problems such as cellular heterogeneity and low access to rare biological materials. In this review， we provide an overview of recent progresses within key single-cell technologies， and focus on their applications in stem cell research and regeneration medicine. We present current developments within these areas and critically discuss how to use singlecell approaches to look for specific stem-cell subpopulation in tissue repair and disease， and to investigate the underlying cellular and molecular mechanisms. Finally we highlight high-throughput screening methods to identify disease-specific and cell-specific drugs using single-cell based two-dimensional and three-dimensional in vitro model systems.

CSTR: 32200.14.cjcb.2019.01.0015