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Role of with No Lysine Kinase 4 in Liquid Transport of Mouse Tracheal Epithelial Cells
Liu Hongfei, Hou Yapeng, Zhou Zhiyu, Zhang Honglei, Ding Yan, Nie Hongguang*
Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang 110122, China
Abstract: The aim of this work was to investigate the regulation of with no lysine kinase 4 (WNK4) on the fluid transport of mouse tracheal epithelial cells. The siRNA-specific silencing of WNK4 gene was used in primary cultured mouse tracheal epithelial cells， and its silencing efficiency was verified by semiquantitative PCR and Western blot. Subsequently， the effect of low expression of the kinase on short-circuit current and Na+ channel α-subunit protein expression in mouse tracheal epithelial cells was recorded by using chamber apparatus and Western blot. Semi-quantitative PCR and Western blot results showed that the siRNA sequence used in this study could silence the expression of WNK4. Using chamber apparatus and Western blot showed that the expression of amiloride-sensitive current and Na+ channel α-subunit protein in mouse tracheal epithelial cells were significantly increased after silencing the kinase. In conclusion， decreasing the expression of WNK4 gene could increase the expression of epithelial Na+ channel α-subunit protein in mouse tracheal epithelial cells and promote Na+ transport， which might be involved in the repair of related edematous lung diseases.
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