The Role of Peroxiredoxin V in Cisplain-Induced Human Liver Cancer Cell Line HepG2 Apoptosis

Abstract: Peroxiredoxin V (Prx V) is a member of the Peroxiredoxin families (Prxs) and plays a role on scavenging the intracellular reactive oxygen species (ROS). In the current study， we observed the regulatory role of Prx V on the apoptosis of HepG2 human liver cancer cells induced by cisplatin (CDDP). The CDDP induced cellular ROS， apoptosis and apoptosis related proteins expression levels were measured by fluorescence microscopy， flow cytometry and Western blot analysis. The results indicated that cisplatin could induce the HepG2 cell apoptosis by accumulating the intracellular ROS levels， while the protein expression level of Prx V was down-regulated. After over-expressed of the Prx V gene with lentivirus， the CDDP induced HepG2 cell apoptosis was decreased in Prx V over-expressed cells compared with that of mock cells. The pro-apototic proteins， such as cleavage-Caspase-3， Bad and cleavage-PARP expression levels were also significantly down-regulated in Prx V (O/V) cells than Mock cells. Our findings demonstrated the protective role of Prx V on CDDP induced HepG2 cell apoptosis and gave a new sight to treatment of the liver cancers.

CSTR: 32200.14.cjcb.2019.01.0011