In Vitro Culture of Bovine Hepatocytes Cells and Preliminary Identification of Subculture Cells

Feng Zhenyue¹, Liu Defu², Song Baifen¹, Fan Zhaowei¹, Cui Yudong¹*

¹College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; ²Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163000, China

Abstract:

Collagenase perfusion method was used to isolate the cells from bovine hepatocytes， in DMEM medium supplemented with 10% fetal bovine serum (FBS)， continuous primary and subculture in vitro and proliferation and morphological changes during cell culture was observed. The chromosome and cell growth curve of the12th passage of the cells were analyzed， at the same time， Glycogen was tested by periodic acid schiff (PAS). The results showed that the population doubling time for the 12th passage of the cell line was 62.8 h. There were 60 chromosomes and a large number of glycogen granules were observed in the cytoplasm PAS staining.

CSTR: 32200.14.cjcb.2019.01.0009